Track 4-1-2: Breeding Cultivated Forage Species for Biomass, Quality and Stress Tolerance

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High temperature over the growth threshold of plants can cause their metabolic and developmental changes, and consequently bring about their slow growth (Lobell et al., 2008). Alfalfa (Medicago sativa L.) is a high-quality legume suitable for growing in semi-arid areas. Its poor thermo tolerance restricts its introduction and popularization in southern China and transitional zone. Fall dormancy is an important indicator for planting planning of alfalfa (Barnes et al., 1977. Existing research shows that alfalfa varieties with a higher fall dormancy rate (FDR) have a stronger heat resistance (Lu, 1998). M. sativa L. ‘Deqin’ (hereinafter referred to as ‘Deqin’) is a wild type alfalfa with a low FDR (1.2) which can grow and proliferate normally in dry-hot valley regions in Deqin County, Yunnan, China. It shows a strong heat resistance. This phenomenon is contradictory to the inference that alfalfa varieties with a higher FDR have a stronger heat resistance. We intend to study physiological and biochemical changes and the change of mRNA expression quantity of differential protein and coding differential protein of ‘Deqin’ under high-temperature stress and analyze heat resistant physiology and molecular biological mechanism of ‘Deqin’. This result will enrich the study theory of alfalfa resources in China and provide theoretical basis for the introduction and popularization of alfalfa with the indicator of fall dormancy rate.

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Study on Protein Related to Heat Tolerance of Medicago sativa L. 'Deqin'

High temperature over the growth threshold of plants can cause their metabolic and developmental changes, and consequently bring about their slow growth (Lobell et al., 2008). Alfalfa (Medicago sativa L.) is a high-quality legume suitable for growing in semi-arid areas. Its poor thermo tolerance restricts its introduction and popularization in southern China and transitional zone. Fall dormancy is an important indicator for planting planning of alfalfa (Barnes et al., 1977. Existing research shows that alfalfa varieties with a higher fall dormancy rate (FDR) have a stronger heat resistance (Lu, 1998). M. sativa L. ‘Deqin’ (hereinafter referred to as ‘Deqin’) is a wild type alfalfa with a low FDR (1.2) which can grow and proliferate normally in dry-hot valley regions in Deqin County, Yunnan, China. It shows a strong heat resistance. This phenomenon is contradictory to the inference that alfalfa varieties with a higher FDR have a stronger heat resistance. We intend to study physiological and biochemical changes and the change of mRNA expression quantity of differential protein and coding differential protein of ‘Deqin’ under high-temperature stress and analyze heat resistant physiology and molecular biological mechanism of ‘Deqin’. This result will enrich the study theory of alfalfa resources in China and provide theoretical basis for the introduction and popularization of alfalfa with the indicator of fall dormancy rate.