Description
The study was carried out on three annual Medicago species (M. polymorpha, M. aculeata and M. ciliaris) each represented by five populations. Cold tolerance of the accessions was tested using a laboratory chilling test on seedling growth and soluble protein content of acclimated and non-acclimated seedlings. Lots of 3-d-old seedlings were submitted to cold treatment for 48 and 96 hours. For each treatment, response was determined by measuring seedlings’ weight and height, and radicle length for both acclimated and non-acclimated seedling lots. Seedling growth was adversely affected by low temperature, and resistant cultivars had a better rate of seedling growth than sensitive cultivars. M. aculeata genotypes were more resistant than those of M. polymorpha and M. sativa cv. Orca, and M. ciliaris populations were intermediate.
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Cold Hardiness Variation in Annual Medicago Species at the Seedling Stage
The study was carried out on three annual Medicago species (M. polymorpha, M. aculeata and M. ciliaris) each represented by five populations. Cold tolerance of the accessions was tested using a laboratory chilling test on seedling growth and soluble protein content of acclimated and non-acclimated seedlings. Lots of 3-d-old seedlings were submitted to cold treatment for 48 and 96 hours. For each treatment, response was determined by measuring seedlings’ weight and height, and radicle length for both acclimated and non-acclimated seedling lots. Seedling growth was adversely affected by low temperature, and resistant cultivars had a better rate of seedling growth than sensitive cultivars. M. aculeata genotypes were more resistant than those of M. polymorpha and M. sativa cv. Orca, and M. ciliaris populations were intermediate.
