Publication Date
1993
Description
The coat protein genes of the potexviruses white clover mosaic virus (WClMY) or potato virus X (PVX) were introduced into Nicotiana benthamiana plants by gene transfer. Transgenic plants expressing the coal protein gene of WCIMY or PYX were highly resistant to infection when challenge-inoculated with the virus from which the coat protein gene was obtained (homologous challenge). Similar resistance was obtained when homologous viral RNA was used as inoculum, The transgenic plants were also protected against infection with the heterologous virus, but only at low inoculum concentrations. The level of protection in PYX transgenic plants was directly correlated with the level of expression of the PYX CP gene.
Citation
Beck, D L.; Van Dolleweerd, C J.; Dudas, B; White, D R.W; and Forster, R.L S., "Coat Protein-Mediated Protection Against White Clover Mosaic Virus and Potato Virus X in Tobacco" (2024). IGC Proceedings (1993-2023). 2.
https://uknowledge.uky.edu/igc/1993/session32/2
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Coat Protein-Mediated Protection Against White Clover Mosaic Virus and Potato Virus X in Tobacco
The coat protein genes of the potexviruses white clover mosaic virus (WClMY) or potato virus X (PVX) were introduced into Nicotiana benthamiana plants by gene transfer. Transgenic plants expressing the coal protein gene of WCIMY or PYX were highly resistant to infection when challenge-inoculated with the virus from which the coat protein gene was obtained (homologous challenge). Similar resistance was obtained when homologous viral RNA was used as inoculum, The transgenic plants were also protected against infection with the heterologous virus, but only at low inoculum concentrations. The level of protection in PYX transgenic plants was directly correlated with the level of expression of the PYX CP gene.
