Publication Date
1993
Description
The effecls of 1-napthaleneacetic acid (NAA) and 6-benzylamino purine (BAP) on callus induction, callus proliferation and plant regeneration were examined ip sainfojn (011ob1ycl1is viciifo/ia Scop.) from differen! ex plant. Then the effects of indole-3-butyric acid (IBA) on root formation from differentiated shoot from calli were examined. The combinations of 1-10 µM ofNAA an4 BAP were better for sainfoin culture. For callus induction, there was no significant difference among 3 explants, but Chere were interactions between explant and media. For plant regeneration, hypocotyl and root are ter explants than cotyledon. The effects of NAA and BAP showed less effective on callus induction and proliferation, but much better on shoot foration °than thoe of 2,4- D and BAP. IBA plays an important role for root formation. The best concentration of IBA was 2S µM.
Citation
Hiroshi, Nakashima and Zhuomeng, Yang, "Tissue Culture of Sainfoin" (2024). IGC Proceedings (1993-2023). 3.
https://uknowledge.uky.edu/igc/1993/session27/3
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Tissue Culture of Sainfoin
The effecls of 1-napthaleneacetic acid (NAA) and 6-benzylamino purine (BAP) on callus induction, callus proliferation and plant regeneration were examined ip sainfojn (011ob1ycl1is viciifo/ia Scop.) from differen! ex plant. Then the effects of indole-3-butyric acid (IBA) on root formation from differentiated shoot from calli were examined. The combinations of 1-10 µM ofNAA an4 BAP were better for sainfoin culture. For callus induction, there was no significant difference among 3 explants, but Chere were interactions between explant and media. For plant regeneration, hypocotyl and root are ter explants than cotyledon. The effects of NAA and BAP showed less effective on callus induction and proliferation, but much better on shoot foration °than thoe of 2,4- D and BAP. IBA plays an important role for root formation. The best concentration of IBA was 2S µM.
