Publication Date
1993
Description
. Callus initiation and plant regeneration from the new intergeneric hybrid of Agropyron repens L., Beauv, x Bromus inermis.1 Leyss. cv. Nanus (Agromus) was carried out. For callus initiation, the inflorescence primordia were incubated on an optimised nutritive medium designated medium-F, supplemented with 2,4-dichlorophenoxyacetic acid (0.5 mg/ I). Within 21 days of plating, embryogenic callus with globular structures were developed, From the 21st day of culture, one-step plant regeneration occurred from callus without subculture. Non-regenerative calli were subcultured to medium-F supplemented with different cytokinins. The cytological analysis of the calli showed wide genetic:il variability in chromosome number with cells at different ploidy level from haploid (n) to triploid (3n) chromosome sets.
Citation
Heszky, L E.; Janovsky, J; Gyulai, G; Kiss, E; and Hangyel, L T., "Application of the Somaclone Method in Fertility Restoration of the Partially Sterile Hybrid of Agropyron repens x Bromus inermis: Callus Initiation and Plant Regeneration" (2024). IGC Proceedings (1993-2023). 1.
https://uknowledge.uky.edu/igc/1993/session27/1
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Application of the Somaclone Method in Fertility Restoration of the Partially Sterile Hybrid of Agropyron repens x Bromus inermis: Callus Initiation and Plant Regeneration
. Callus initiation and plant regeneration from the new intergeneric hybrid of Agropyron repens L., Beauv, x Bromus inermis.1 Leyss. cv. Nanus (Agromus) was carried out. For callus initiation, the inflorescence primordia were incubated on an optimised nutritive medium designated medium-F, supplemented with 2,4-dichlorophenoxyacetic acid (0.5 mg/ I). Within 21 days of plating, embryogenic callus with globular structures were developed, From the 21st day of culture, one-step plant regeneration occurred from callus without subculture. Non-regenerative calli were subcultured to medium-F supplemented with different cytokinins. The cytological analysis of the calli showed wide genetic:il variability in chromosome number with cells at different ploidy level from haploid (n) to triploid (3n) chromosome sets.
