Determining the Effects of CD151 and β₁ on Tumor Cell Adhesion and Migration

Author

Rachel R. Essex, University of KentuckyFollow

Date Available

12-10-2015

Year of Publication

2015

Degree Name

Master of Science in Chemical Engineering (MSChE)

Document Type

Master's Thesis

College

Engineering

Department/School/Program

Chemical and Materials Engineering

First Advisor

Dr. Kimberly Anderson

Abstract

Previous studies have shown that the upregulation of CD151 and β₁ is associated with poor prognosis in many cancers such as breast cancer. Studies have provided evidence that these proteins are associated with the adhesion and migration of tumor cells. In this study, a microfluidic flow chamber was utilized to determine how CD151 and β₁ affected the firm and initial adhesion of metastatic breast cancer cells to a planar endothelial monolayer under shear stress. This system mimicked the adhesion of metastatic breast cancer cells to the endothelial cells of the circulatory system. CD151 and β₁ increased the firm adhesion of metastatic breast cancer cells onto an endothelial monolayer when subjected to high shear stresses. CD151 and β₁ increased the initial adhesion of metastatic breast cancer cells onto an endothelial monolayer. A transwell assay was utilized to determine how CD151 and β₁ affected random migration through different matrixes and random transendothelial migration. CD151 and β₁ decreased the random migration of metastatic breast cancer cells through matrices. Additionally, background information is provided related to the metastatic cascade, how it can be modeled with microfluidics, and how CD151 and β₁ have been known to effect cancer and metastasis.

Recommended Citation

Essex, Rachel R., "Determining the Effects of CD151 and β₁ on Tumor Cell Adhesion and Migration" (2015). Theses and Dissertations--Chemical and Materials Engineering. 56.
https://uknowledge.uky.edu/cme_etds/56