In the female gamete of flowering plants, sperm nuclear migration is controlled by a constant inward movement of actin filaments (F-actin) for successful fertilization. This dynamic F-actin movement is ARP2/3-independent, raising the question of how actin nucleation and polymerization is controlled in the female gamete. Using confocal microscopy live-cell imaging in combination with a pharmacological approach, we assessed the involvement of another group of actin nucleators, formins, in F-actin inward movement in the central cell of Arabidopsis thaliana. We identify that the inhibition of the formin function, by formin inhibitor SMIFH2, significantly reduced the dynamic inward movement of F-actin in the central cell, indicating that formins play a major role in actin nucleation required for F-actin inward movement in the central cell.

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Published in Plant Signaling & Behavior, v. 16, issue 8.

This is an Accepted Manuscript version of the following article, accepted for publication in Plant Signaling & Behavior. Ali, M. F., & Kawashima, T. (2021). Formins control dynamics of F-actin in the central cell of Arabidopsis thaliana. Plant Signaling & Behavior, 16(8), 1920192. https://doi.org/10.1080/15592324.2021.1920192

It is deposited under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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This work was supported by the National Science Foundation [IOS-1928836]; National Institute of Food and Agriculture [1014280].