In the female gamete of flowering plants, sperm nuclear migration is controlled by a constant inward movement of actin filaments (F-actin) for successful fertilization. This dynamic F-actin movement is ARP2/3-independent, raising the question of how actin nucleation and polymerization is controlled in the female gamete. Using confocal microscopy live-cell imaging in combination with a pharmacological approach, we assessed the involvement of another group of actin nucleators, formins, in F-actin inward movement in the central cell of Arabidopsis thaliana. We identify that the inhibition of the formin function, by formin inhibitor SMIFH2, significantly reduced the dynamic inward movement of F-actin in the central cell, indicating that formins play a major role in actin nucleation required for F-actin inward movement in the central cell.
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This work was supported by the National Science Foundation [IOS-1928836]; National Institute of Food and Agriculture .
Ali, Mohammad F. and Kawashima, Tomokazu, "Formins Control Dynamics of F-Actin in the Central Cell of Arabidopsis thaliana" (2021). Plant and Soil Sciences Faculty Publications. 157.
Available for download on Wednesday, May 04, 2022