Objective: Nanoharvesting from intact plants, organs, and cultured cells is a method in which nanoparticles are co-incubated with the target tissue, which leads to the internalization of nanoparticles. Internalized nanoparticles are coated in situ with specific metabolites that form a dynamic surface layer called a bio-corona. Our previous study showed that metabolites that form the bio-corona around anatase TiO2 nanoparticles incubated with leaves of the model plant Arabidopsis thaliana are enriched for flavonoids and lipids. The present study focused on the identification of metabolites isolated by nanoharvesting from two medicinal plants, Ocimum sanctum (Tulsi) and Rubia tinctorum (common madder).
Results: To identify metabolites that form the bio-corona, Tulsi leaves and madder roots were incubated with ultra-small anatase TiO2 nanoparticles, the coated nanoparticles were collected, and the adsorbed molecules were released from the nanoparticle surface and analyzed using an untargeted metabolomics approach. Similar to the results in which Arabidopsis tissue was used as a source of metabolites, TiO2 nanoparticle bio-coronas from Tulsi and madder were enriched for flavonoids and lipids, suggesting that nanoharvesting has a wide-range application potential. The third group of metabolites enriched in bio-coronas isolated from both plants were small peptides with C-terminal arginine and lysine residues.
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This work was funded by the USDA National Institute of Food and Agriculture competitive grants program project 2015-67021-22997 and by the Kentucky Tobacco Research and Development Center (KTRDC).
All research materials are commercially available and all data is available upon request.
Descriptions of the additional files:
Additional file 1: Table S1. List of significant metabolites enriched in bio-coronas of NPs isolated from Tulsi leaves. Names of the compounds, their chemical class according to the Human Metabolome Database (HMDB, http://www.hmdb.ca/ ) classification, and chemical class coding used for hierarchical clustering analysis shown in Fig. 1b are shown.
Additional file 2: Table S2. List of significant metabolites enriched in bio-coronas of NPs isolated from madder roots. Names of the compounds, their chemical class according to the Human Metabolome Database (HMDB, http://www.hmdb.ca/ ) classification, and chemical class coding used for hierarchical clustering analysis shown in Fig. 2c are shown.
Kurepa, Jasmina and Smalle, Jan A., "Composition of the Metabolomic Bio-Coronas Isolated from Ocimum sanctum and Rubia tinctorum" (2021). Plant and Soil Sciences Faculty Publications. 153.