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University of Kentucky Libraries
Approximately 40 tall fescue tillers were randomly collected and frozen from each of the 20 treatment plots.
Tillers were cut at 7.6 cm above ground level and tested for the presence of the Epichloe endophyte using an enzyme-linked immunosorbent assay.
Tillers from each plot were sorted into 'infected' vs 'uninfected' groups, lyophilized, and ground through a 1mm screen using a Cyclotec 1093 mill.
Ground material from the endophyte infected tillers was analyzed for ergot and loline alkaloids in the lab of Lowell Bush at the University of Kentucky, Plant and Soil Sciences Dept.
For details on alkaloid analyses see: McCulley et al. Oct. 2014. Frontiers in Chemistry, Volume 2, Article 88 (www.frontiersin.org -- doi: 10.3389/fchem.2014.00088)
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This dataset is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided that the dataset creator and source are credited and that changes (if any) are clearly indicated.
Details about the experiment are on the first tab of the downloadable file.
NICCR plots, University of Kentucky Spindletop Research Farm
Geographic coordinates: 38.103812, -84.488871
2009 to 2013
This work was supported by a grant to Rebecca L. McCulley (US Department of Energy 08-SC-NICCR-1073), a cooperative agreement with the USDA-ARS Forage Animal Production Research Unit (58-6440-7-135), the Kentucky Agricultural Experiment Station (KY006045), and the University of Kentucky's College of Agriculture, Food, and the Environment.
McCulley, R. L., Bush, L. P., Carlisle, A. E., Ji, H, & Nelson, J. A. (2014). Warming reduces tall fescue abundance but stimulates toxic alkaloid concentrations in transition zone pastures of the U.S. Frontiers in Chemistry, 2(88). https://doi.org/10.3389/fchem.2014.00088
Click here to access this article from UKnowledge.
McCulley, R. L. (2017). Ergot and loline alkaloid concentrations in endophyte-infected tall fescue tillers. University of Kentucky Libraries. https://doi.org/10.13023/K2RP48