Forage Climate Change Experiment Research Data
Researcher ORCID Identifier
Dataset Creation Date
6-2009
Release Date
2-2017
Publisher
University of Kentucky Libraries
Description
Approximately 40 tall fescue tillers were randomly collected and frozen from each of the 20 treatment plots.
Tillers were cut at 7.6 cm above ground level and tested for the presence of the Epichloe endophyte using an enzyme-linked immunosorbent assay.
Tillers from each plot were sorted into 'infected' vs 'uninfected' groups, lyophilized, and ground through a 1mm screen using a Cyclotec 1093 mill.
Ground material from the endophyte infected tillers was analyzed for ergot and loline alkaloids in the lab of Lowell Bush at the University of Kentucky, Plant and Soil Sciences Dept.
For details on alkaloid analyses see: McCulley et al. Oct. 2014. Frontiers in Chemistry, Volume 2, Article 88 (www.frontiersin.org -- doi: 10.3389/fchem.2014.00088)
Digital Object Identifier (DOI)
https://doi.org/10.13023/K2RP48
Rights
This dataset is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided that the dataset creator and source are credited and that changes (if any) are clearly indicated.
Supporting Information
Details about the experiment are on the first tab of the downloadable file.
File Format
Excel file
File Size
26 KB
Spatial Coverage
NICCR plots, University of Kentucky Spindletop Research Farm
Geographic coordinates: 38.103812, -84.488871
Temporal Coverage
2009 to 2013
Language
English
Funding Information
This work was supported by a grant to Rebecca L. McCulley (US Department of Energy 08-SC-NICCR-1073), a cooperative agreement with the USDA-ARS Forage Animal Production Research Unit (58-6440-7-135), the Kentucky Agricultural Experiment Station (KY006045), and the University of Kentucky's College of Agriculture, Food, and the Environment.
Related Content
McCulley, R. L., Bush, L. P., Carlisle, A. E., Ji, H, & Nelson, J. A. (2014). Warming reduces tall fescue abundance but stimulates toxic alkaloid concentrations in transition zone pastures of the U.S. Frontiers in Chemistry, 2(88). https://doi.org/10.3389/fchem.2014.00088
Click here to access this article from UKnowledge.
Recommended Citation
McCulley, R. L. (2017). Ergot and loline alkaloid concentrations in endophyte-infected tall fescue tillers. University of Kentucky Libraries. https://doi.org/10.13023/K2RP48
Supporting information in non-proprietary format
Alkaloids-data.csv (8 kB)
Research data in non-proprietary format
Notes
The research data and supporting information are also available in a non-proprietary format and are available for download as the additional files listed below.