Forage Climate Change Experiment Research Data

Researcher ORCID Identifier

Dataset Creation Date


Release Date



University of Kentucky Libraries


Approximately 40 tall fescue tillers were randomly collected and frozen from each of the 20 treatment plots.

Tillers were cut at 7.6 cm above ground level and tested for the presence of the Epichloe endophyte using an enzyme-linked immunosorbent assay.

Tillers from each plot were sorted into 'infected' vs 'uninfected' groups, lyophilized, and ground through a 1mm screen using a Cyclotec 1093 mill.

Ground material from the endophyte infected tillers was analyzed for ergot and loline alkaloids in the lab of Lowell Bush at the University of Kentucky, Plant and Soil Sciences Dept.

For details on alkaloid analyses see: McCulley et al. Oct. 2014. Frontiers in Chemistry, Volume 2, Article 88 ( -- doi: 10.3389/fchem.2014.00088)

Digital Object Identifier (DOI)


This dataset is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided that the dataset creator and source are credited and that changes (if any) are clearly indicated.

Supporting Information

Details about the experiment are on the first tab of the downloadable file.

File Format

Excel file

File Size

26 KB

Spatial Coverage

NICCR plots, University of Kentucky Spindletop Research Farm

Geographic coordinates: 38.103812, -84.488871

Temporal Coverage

2009 to 2013



Funding Information

This work was supported by a grant to Rebecca L. McCulley (US Department of Energy 08-SC-NICCR-1073), a cooperative agreement with the USDA-ARS Forage Animal Production Research Unit (58-6440-7-135), the Kentucky Agricultural Experiment Station (KY006045), and the University of Kentucky's College of Agriculture, Food, and the Environment.

Related Content

McCulley, R. L., Bush, L. P., Carlisle, A. E., Ji, H, & Nelson, J. A. (2014). Warming reduces tall fescue abundance but stimulates toxic alkaloid concentrations in transition zone pastures of the U.S. Frontiers in Chemistry, 2(88).

Click here to access this article from UKnowledge.


The research data and supporting information are also available in a non-proprietary format and are available for download as the additional files listed below.

Alkaloids-readme.csv (1 kB)
Supporting information in non-proprietary format

Alkaloids-data.csv (8 kB)
Research data in non-proprietary format