US Patent Number
The Bacterial cocaine esterase (CocE) mutants disclosed herein each have enhanced catalytic efficiency for ( -)cocaine, as compared to CocE mutants in the prior art, including CocE mutant E172-173. The presently-disclosed subject matter further includes a pharmaceutical composition including a mutant of bacterial cocaine hydrolase, as described herein, and a suitable pharmaceutical carrier. The presently-disclosed subject matter further includes a method of treating a cocaine-induced condition comprising administering to an individual an effective amount of a mutant of bacterial cocaine hydrolase variant, as disclosed herein, to accelerate cocaine metabolism and produce biologically inactive metabolites.
University of Kentucky Research Foundation, Lexington, KY (US)
Zhan, Chang-Guo; Zheng, Fang; and Fang, Lei, "High Activity Mutants of Cocaine Esterase for Cocaine Hydrolysis" (2018). Pharmaceutical Sciences Faculty Patents. 172.