US Patent Number
Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. Specifically, some embodiments of the invention relate to highly efficient, thermostable, and long-lasting cocaine esterase (CocE) mutants that can protect against the toxic and reinforcing effects of cocaine in subjects. Provided herein are mutant CocE polypeptides displaying thermostable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need via administration of mutant CocE as well as methods for high-throughput screening of candidate esterase polypeptides.
The Trustees of Columbia University in the City of New York, New York, NY (US), The Regents of the University of Michigan, Ann Arbor, MI (US), University of Kentucky Research Foundation, Lexington, KY (US)
Landry, Donald W.; MacDonald, Joanne; Deng, Shi-Xiang; Zhan, Chang-Guo; Gao, Daquan; Woods, James H.; Sunahara, Roger K.; Narasimhan, Diwahar L.; Yang, Victor; Ko, Mei-Chuan Holden; Tesmer, John J.; Lee, Tien-Yi; and Kwon, Young Min, "Anti-Cocaine Compositions and Treatment" (2012). Pharmaceutical Sciences Faculty Patents. 12.