Genome Sequences of Three Phytopathogenic Species of the Magnaporthaceae Family of Fungi

Authors

Laura H. Okagaki, North Carolina State University
Cristiano C. Nunes, North Carolina State University
Joshua Sailsbery, North Carolina State University
Brent Clay, North Carolina State University
Doug Brown, North Carolina State University
Titus John, North Carolina State University
Yeonyee Oh, North Carolina State University
Nelson Young, University of Massachusetts - Amherst
Michael Fitzgerald, The Broad Institute of MIT and Harvard
Brian J. Haas, The Broad Institute of MIT and Harvard
Qiandong Zeng, The Broad Institute of MIT and Harvard
Sarah Young, The Broad Institute of MIT and Harvard
Xian Adiconis, The Broad Institute of MIT and Harvard
Lin Fan, The Broad Institute of MIT and Harvard
Joshua Z. Levin, The Broad Institute of MIT and Harvard
Thomas K. Mitchell, Ohio State University
Patricia A. Okubara, USDA-ARS
Mark L. Farman, University of KentuckyFollow
Linda M. Kohn, University of Toronto, Canada
Bruce Birren, The Broad Institute of MIT and Harvard
Li-Jun Ma, University of Massachusetts - Amherst
Ralph A. Dean, North Carolina State University

Abstract

Magnaporthaceae is a family of ascomycetes that includes three fungi of great economic importance: Magnaporthe oryzae, Gaeumannomyces graminis var. tritici, and Magnaporthe poae. These three fungi cause widespread disease and loss in cereal and grass crops, including rice blast disease (M. oryzae), take-all disease in wheat and other grasses (G. graminis), and summer patch disease in turf grasses (M. poae). Here, we present the finished genome sequence for M. oryzae and draft sequences for M. poae and G. graminis var. tritici. We used multiple technologies to sequence and annotate the genomes of M. oryzae, M. poae, and G. graminis var. tritici. The M. oryzae genome is now finished to seven chromosomes whereas M. poae and G. graminis var. tritici are sequenced to 40.0× and 25.0× coverage respectively. Gene models were developed by the use of multiple computational techniques and further supported by RNAseq data. In addition, we performed preliminary analysis of genome architecture and repetitive element DNA.

Document Type

Article

Publication Date

12-1-2015

Notes/Citation Information

Published in G3: Genes, Genomes, Genetics, v. 5, no. 12, p. 2539-2545.

Copyright © 2015 Okagaki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Digital Object Identifier (DOI)

http://dx.doi.org/10.1534/g3.115.020057

Funding Information

Laura Okagaki was supported by the Triinstitutional Molecular Mycology and Pathogenesis training grant (National Institutes of Health 5T32-AI052080-09). Funding was provided by National Science Foundation MCB-0333269 (Birren and Dean), Current Research Information System Project 5348- 22000-012-00D (Okubara), and MCB-0731808 (Dean, Ma, Kohn, Okubara, and Mitchell).

Repository Citation

Okagaki, Laura H.; Nunes, Cristiano C.; Sailsbery, Joshua; Clay, Brent; Brown, Doug; John, Titus; Oh, Yeonyee; Young, Nelson; Fitzgerald, Michael; Haas, Brian J.; Zeng, Qiandong; Young, Sarah; Adiconis, Xian; Fan, Lin; Levin, Joshua Z.; Mitchell, Thomas K.; Okubara, Patricia A.; Farman, Mark L.; Kohn, Linda M.; Birren, Bruce; Ma, Li-Jun; and Dean, Ralph A., "Genome Sequences of Three Phytopathogenic Species of the Magnaporthaceae Family of Fungi" (2015). Plant Pathology Faculty Publications. 50.
https://uknowledge.uky.edu/plantpath_facpub/50