Author ORCID Identifier
https://orcid.org/0000-0002-9112-9410
Date Available
1-10-2025
Year of Publication
2025
Document Type
Master's Thesis
Degree Name
Master of Science (MS)
College
Pharmacy
Department/School/Program
Pharmaceutical Sciences
Faculty
Dr. Esther Penni Black
Faculty
Dr. David Feola
Faculty
Dr. David Feola
Abstract
Protein-protein interactions are critical in cellular signaling pathways. This project investigates binding interactions between SHP-2, a tyrosine phosphatase, and DDX3, an RNA helicase. There is a specific focus on the impact that gain-of-function SHP-2 mutations may impact binding to DDX3 via conformational change. Using immunoprecipitation protocols as well as western blotting we were able to provide insights into SHP-2 molecular mechanisms and subsequent binding to DDX3.
Digital Object Identifier (DOI)
https://doi.org/10.13023/etd.2024.535
Recommended Citation
Montgomery, Devin, "USING HYPERACTIVE SHP-2 MUTANTS, A PHOSPHATASE, TO IDENTIFY INTERACTIONS WITH THE RNA HELICASE DDX3" (2025). Theses and Dissertations--Pharmacy. 165.
https://uknowledge.uky.edu/pharmacy_etds/165
