US Patent Number
A method for obtaining molecules from a substantially intact single cell is disclosed. The method differs from prior methods that obtain a variable fraction of a cell's contents and therefore cannot quantitatively estimate the number of molecules in the cells. The present method comprises isolating and harvesting a substantially intact single cell from its organ tissue comprising the steps of subjecting a tissue mass to a dissociation method so that the cells are dissociated from the tissue to expose cell bodies or cell processes, contacting a dissociated cell with a device capable of collecting the cell from the tissue substantially intact, withdrawing device with the cell attached, and then isolating or detecting the molecules in the single cell.
University of Kentucky Research Foundation, Lexington, KY (US)
Landfield, Philip W.; Thibault, Olivier; Blalock, Eric M.; Chen, Kuey-Chu; and Kaminker, Patrick, "Method for Analyzing Molecular Expression or Function in an Intact Single Cell" (2002). Pharmacology and Nutritional Sciences Faculty Patents. 44.