Previous studies implicate the nonreceptor protein tyrosine kinase (PTK) p59fyn in the propagation of signals from the B cell antigen receptor. To elucidate the functions of this kinase, we examined B cell responsiveness in mice engineered to lack the hematopoietic isoform of p59fyn. Remarkably, antigen receptor signaling was only modestly defective in fynTnull B cells. In contrast, signaling from the interleukin (IL)-5 receptor which ordinarily provides a comitogenic stimulus with antiimmunoglobulin, was completely blocked. Our results document the importance of p59fynT in IL-5 responses in B cells, and they support a general model for cytokine receptor signal transduction involving the simultaneous recruitment of at least three families of PTK.
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This work was supported in part by a grant from the National Institutes of Health (CA45682).
Appleby, Mark W.; Kerner, James D.; Chien, Sylvia; Maliszewski, Charles R.; Bondada, Subbarao; and Perlmutter, Roger M., "Involvement of p59fynT in Interleukin-5 Receptor Signaling" (1995). Microbiology, Immunology, and Molecular Genetics Faculty Publications. 121.