Abstract

Monoclonal antibodies are powerful and versatile tools that enable the study of proteins in diverse contexts. They are often utilized to assist with identification of subcellular localization and characterization of the function of target proteins of interest. However, because there can be considerable sequence diversity between orthologous proteins in Xenopus and mammals, antibodies produced against mouse or human proteins often do not recognize Xenopus counterparts. To address this issue, we refined existing mouse monoclonal antibody production protocols to generate antibodies against Xenopus proteins of interest. Here, we describe several approaches for the generation of useful mouse anti-Xenopus antibodies to multiple Xenopus proteins and their validation in various experimental approaches. These novel antibodies are now available to the research community through the Developmental Study Hybridoma Bank (DSHB).

Document Type

Article

Publication Date

2-2023

Notes/Citation Information

© 2023. Published by The Company of Biologists Ltd

Digital Object Identifier (DOI)

https://doi.org/10.1242/dev.201309

Funding Information

This work was supported by a grant from the National Institutes of Health (R24OD021485 from the office of the directorate to D.A.). D.A. is also supported by grants from the National Institute of Dental and Craniofacial Research (R01DE016289 to D.A. and R01DE026434 to S. Moody and D.A.). Deposited in PMC for release after 12 months.

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