Abstract
Oleate hydratase (OhyA), a flavoenzyme that catalyzes the hydration of unsaturated fatty acids, has been identified in various Bacillales organisms, including those in the Listeria, Lysinibacillus, Paenibacillus, and Staphylococcus genera. In this study, we combine structural biology with molecular and phylogenetic analyses to investigate the evolutionary dynamics of the OhyA protein family within the Bacillales order. Our evolutionary analysis reveals two distinct OhyA clades (clade I and clade II) within Bacillales that, while sharing catalytic function, exhibit significant genomic and structural differences. Our findings suggest that these OhyA clades originated from independent evolutionary processes through convergent evolution rather than gene duplication. We also show that the evolutionary divergence in OhyA is likely due to intrinsic sequence variations rather than being strictly linked to functional domain changes. Furthermore, within the Staphylococcus genus, we observed that the evolution of the ohyA gene aligns with the species tree, supporting a common ancestral origin. This study enhances our understanding of the impact of evolutionary history on the structure and function of OhyA across the Bacillales order.
Document Type
Article
Publication Date
12-2024
Digital Object Identifier (DOI)
https://doi.org/10.3389/fmolb.2024.1485485
Funding Information
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This research was supported by NIH grant AI166116 (CR). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Repository Citation
Neff, Robert J.; Lages, Priscilla C.; Donworth, Shannon K.; Brien, James D.; and Radka, Christopher D., "Independent evolution of oleate hydratase clades in Bacillales reflects molecular convergence" (2024). Markey Cancer Center Faculty Publications. 227.
https://uknowledge.uky.edu/markey_facpub/227
Notes/Citation Information
© 2024 Neff, Lages, Donworth, Brien and Radka. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.