Abstract
The purpose of this study was to determine the relative importance of the metabolic effects of insulin for diabetes prevention by administering insulin or an inactive insulin analog by daily subcutaneous injections to prediabetic mice. A recombinant monomeric human insulin analog, which does not bind to the insulin receptor as a consequence of an alteration of a single amino acid at position 25 of the B chain, was shown to be equally effective at diabetes prevention as was intact insulin. In contrast to native insulin, the insulin analog did not cause hypoglycemia after subcutaneous injection. The insulin analog, however, protected young adult mice from diabetes, even when it was initiated after the onset of extensive lymphocytic infiltration of the islets. Thus, preventative therapy by daily subcutaneous injections of insulin does not require the hypoglycemic response, or binding to the insulin receptor to prevent the onset of type I diabetes.
Document Type
Article
Publication Date
9-15-1997
Digital Object Identifier (DOI)
https://doi.org/10.1172/jci119654
Funding Information
D. Karounos is supported in part by the Veterans Affairs Merit Review VMU#92-0011V, the Greenwall Foundation, and the University of Kentucky Medical Center Physician Scientist Award.
Repository Citation
Karounos, Dennis G.; Bryson, J. Scott; and Cohen, D. A., "Metabolically Inactive Insulin Analog Prevents Type I Diabetes in Prediabetic NOD Mice" (1997). Internal Medicine Faculty Publications. 258.
https://uknowledge.uky.edu/internalmedicine_facpub/258
Notes/Citation Information
Published in The Journal of Clinical Investigation, v. 100, no. 6.
© 1997 The American Society for Clinical Investigation
The copyright holder has granted the permission for posting the article here.