The intestinal epithelium of the gastrointestinal (GI) tract constantly renews itself to absorb nutrients and provide protection for the body from the outside world. Since the intestinal epithelium is constantly exposed to various chemicals and dietary components, it is critical to determine which constituents promote or inhibit intestinal epithelium health and growth rate. Intestinal organoids, three-dimensional miniature models of the intestines, represent an ex vivo tool to investigate intestinal physiology and growth patterns. In this study, we measured the growth rates of murine intestinal organoids exposed to various concentrations of different dietary constituents. Results indicate that caffeic acid inhibited organoid growth in a concentration-dependent manner, curcumin exhibited variable effectiveness, and vitamin C had no effect on organoid growth.

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Published in PLOS ONE, v. 13, no. 2, e0191517, p. 1-14.

© 2018 Cai et al.

This is an openaccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Dr. Wang is grateful for the support from Crohn’s & Colitis Foundation of America (CCFA) Career Award (No. 348137), National Science Foundation (No. EEC 1560012), PhRMA Foundation Research Starter Award (No. RSGTMT17), and McGee-Wagner Interdisciplinary Research Foundation. The research was also supported (TAB) by NIH (2RO1 DK095662) and VA Merit (1I01CX001353) grants.

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S1 Table. Methods for dissolving the various constituents used in this study. https://doi.org/10.1371/journal.pone.0191517.s001 (TIF)

journal.pone.0191517.s001.tif (228 kB)
S1 Table. Methods for dissolving the various constituents used in this study.