Animal studies demonstrate that hyperlipidemia and renal lipid accumulation contribute to the pathogenesis of diabetic nephropathy (DN). We previously demonstrated that renal lipoproteins colocalize with biglycan, a renal proteoglycan. The purpose of this study was to determine whether prevention of renal lipid (apoB) accumulation attenuates DN. Biglycan-deficient and biglycan wild-type Ldlr−/− mice were made diabetic via streptozotocin and fed a high cholesterol diet. As biglycan deficiency is associated with elevated transforming growth factor-β (TGF-β), in some experiments mice were injected with either the TGF-β-neutralizing antibody, 1D11, or with 13C4, an irrelevant control antibody. Biglycan deficiency had no significant effect on renal apoB accumulation, but led to modest attenuation of DN with ∼30% reduction in albuminuria; however, biglycan deficiency caused a striking elevation in TGF-β. Use of 1D11 led to sustained suppression of TGF-β for approximately 8 weeks at a time. The 1D11 treatment caused decreased renal apoB accumulation, decreased albuminuria, decreased renal hypertrophy, and improved survival, compared with the 13C4 treatment. Thus, prevention of renal apoB accumulation is protective against development of DN. Furthermore, this study demonstrates that prevention of renal apoB accumulation is a mechanism by which TGF-β inhibition is nephroprotective.

Document Type


Publication Date


Notes/Citation Information

Published in Journal of Lipid Research, v. 58, issue 12, p. 2264-2274.

This research was originally published in the Journal of Lipid Research. Patricia G. Wilson, Joel C. Thompson, Meghan H. Yoder, Richard Charnigo, and Lisa R. Tannock. Prevention of Renal ApoB Retention is Protective Against Diabetic Nephropathy: Role of TGF-β Inhibition. J. Lipid Res. 2017; 58:2264-2274. © 2017 by the American Society for Biochemistry and Molecular Biology, Inc.

The copyright holder has granted the permission for posting the article here.

Digital Object Identifier (DOI)


Funding Information

This work was supported by US Department of Veterans Affairs Merit Review Award BX000622 to L.R.T. and cores supported by National Institutes of Health Grant P30 GM103527.

Related Content

The online version of this article (available at http://www.jlr.org) contains a supplement.

jlr.M078204-1.pdf (705 kB)
Supplemental Material