Bone marrow-derived progenitor cells are mobilized into the peripheral blood after acute myocardial injury and in chronic ischemic heart disease. However, the mechanisms responsible for this mobilization are poorly understood. We examined the relationship between plasma levels of bioactive lipids and number of circulating progenitor cells (CPCs) in patients (N = 437) undergoing elective or emergent cardiac catheterization. Plasma levels of sphingosine-1 phosphate (S1P) and ceramide-1 phosphate (C1P) were quantified using mass spectrometry. CPCs were assessed using flow cytometry. S1P levels correlated with the numbers of CD34+, CD34+/CD133+, and CD34+/CXCR4+ CPCs even after adjustment for potential confounding factors. However, no significant correlation was observed between C1P levels and CPC count. Plasma levels of S1P correlated with the number of CPCs in patients with coronary artery disease, suggesting an important mechanistic role for S1P in stem cell mobilization. The therapeutic effects of adjunctive S1P therapy to mobilize endogenous stem cells need to be investigated.
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A.K.A.-L. is supported by the University of Kentucky (UK) Clinical and Translational Science Pilot Award Grant UL1TR000117, the UK Center of Biomedical Research Excellence Early Career Program Grant P20 GM103527, and National Institutes of Health (NIH) Grant R56 HL124266. A.A.Q. is supported by NIH Grants 5P01HL101398-02, 1P20HL113451-01, 1R56HL126558-01, 1RF1AG051633-01, R01 NS064162-01, R01 HL89650-01, HL095479-01, 1U10HL110302-01, 1DP3DK094346-01, and 2P01HL086773-06A1. S.S.H. is supported by the Abraham J. and Phyllis Katz Foundation.
Hayek, Salim S.; Klyachkin, Yuri; Asfour, Ahmed; Ghasemzadeh, Nima; Awad, Mosaab; Hesaroieh, Iraj; Ahmed, Hina; Gray, Brandon; Kim, Jinhee; Waller, Edmund K.; Quyyumi, Arshed A.; and Abdel-Latif, Ahmed K., "Bioactive Lipids and Circulating Progenitor Cells in Patients with Cardiovascular Disease" (2017). Internal Medicine Faculty Publications. 118.