Publication Date
1993
Description
Three cellobiohydrolases were highly puriried from the rumen fungus Neocallimastix patriciarun. Two of these enzymes were liberated from insoluble material by extraction with the non-ionic detergent Triton X-114, requiring the presence of detergent or dissociating agent throughout their purification. The native cellobiohydrolase is probably membrane associated.
Citation
Alyward, J H.; Xue, Gang Ping; Simpson, G D.; and Orpin, C G., "Cellobiohydrolase (CBH) from Neocallimastix patriciarum: A Membrane Associated Complex?" (2024). IGC Proceedings (1993-2023). 5.
https://uknowledge.uky.edu/igc/1993/session34/5
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Cellobiohydrolase (CBH) from Neocallimastix patriciarum: A Membrane Associated Complex?
Three cellobiohydrolases were highly puriried from the rumen fungus Neocallimastix patriciarun. Two of these enzymes were liberated from insoluble material by extraction with the non-ionic detergent Triton X-114, requiring the presence of detergent or dissociating agent throughout their purification. The native cellobiohydrolase is probably membrane associated.