Somatic Embryogensis and Plant Regeneration from Long-Term Suspension Culture of Digitaria decumbens
Publication Date
1993
Description
The optimum condition for embryogenic callus formation and plant regeneration from immature inflorescences of Digltarla decumbens was MS medium supplemented with O,S mg/I 2,4-D, 2.0 mg/) kinetin and 0,591, NaCl. For long-term subcultures, pieces of embryogenic callus were cultured on MS liquid medium supplemented with 1-2 mg/1 2,4-D, 0.591, NaCl, 5% coconut milk, SOO mg/I casein hydrolase and O.S mg/I 6-benzylaminopurlne, and were subcultured every 4-S days. Bmbryogenic suspension cultures were maintained for more than 1 year. More than 5000 plants may be produced from an explant within 6 months. Bmbryoids from suspension cultures showed a bipolar organisation.
Citation
Cheng, Yu-Kuei, "Somatic Embryogensis and Plant Regeneration from Long-Term Suspension Culture of Digitaria decumbens" (2024). IGC Proceedings (1993-2023). 8.
https://uknowledge.uky.edu/igc/1993/session27/8
Included in
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Somatic Embryogensis and Plant Regeneration from Long-Term Suspension Culture of Digitaria decumbens
The optimum condition for embryogenic callus formation and plant regeneration from immature inflorescences of Digltarla decumbens was MS medium supplemented with O,S mg/I 2,4-D, 2.0 mg/) kinetin and 0,591, NaCl. For long-term subcultures, pieces of embryogenic callus were cultured on MS liquid medium supplemented with 1-2 mg/1 2,4-D, 0.591, NaCl, 5% coconut milk, SOO mg/I casein hydrolase and O.S mg/I 6-benzylaminopurlne, and were subcultured every 4-S days. Bmbryogenic suspension cultures were maintained for more than 1 year. More than 5000 plants may be produced from an explant within 6 months. Bmbryoids from suspension cultures showed a bipolar organisation.
