Publication Date

1989

Description

The performance of Near Infrared Reflectance Spectroscopy (NIRS) depends critically on the sample set used in the cali­bration process (e.g. Hruschka and Norris, 1987; Paul, 1988). In forage quality testing a particular problem exists in that nar­row based sample sets comprising only single botanical species may give equations of limited value under practical conditions. Broad based sample sets may, however, lead to equations with reduced analytical precision as compared to the former. The present work aims at a spectroscopic interpretation and a statistical validation of NIRS equations for protein and energy concentration developed from broad based and narrow based sets of forage grass and legume calibration samples.

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Set Up on NIRS Calibration Populations for Predicting Protein and Net Energy Content in Forage Grasses and Legumes

The performance of Near Infrared Reflectance Spectroscopy (NIRS) depends critically on the sample set used in the cali­bration process (e.g. Hruschka and Norris, 1987; Paul, 1988). In forage quality testing a particular problem exists in that nar­row based sample sets comprising only single botanical species may give equations of limited value under practical conditions. Broad based sample sets may, however, lead to equations with reduced analytical precision as compared to the former. The present work aims at a spectroscopic interpretation and a statistical validation of NIRS equations for protein and energy concentration developed from broad based and narrow based sets of forage grass and legume calibration samples.