Publication Date

1989

Description

King grass (2n = 28) is widely used in Cuba for forage pro­duction. The increase in the variability of this species is there­fore of special interest in agriculture. Tissue culture as a tech­nique to produce and select somaclones from the king grass donor was used to obtain variability. These were obtained when the apical meristems were cultivated in MS medium with 3 mg/I of 2,4 D to regenerate the plants with different hormonal combinations. Those of 0.1 mg/! of kinetine (Martinez, 1986) were prominent. The variability in the selection and evaluation processes is exposed in this paper.

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Obtention of King Grass (Pennisetum purpureum) Clones from Tissue Culture. Selection and Evaluation of Mutants

King grass (2n = 28) is widely used in Cuba for forage pro­duction. The increase in the variability of this species is there­fore of special interest in agriculture. Tissue culture as a tech­nique to produce and select somaclones from the king grass donor was used to obtain variability. These were obtained when the apical meristems were cultivated in MS medium with 3 mg/I of 2,4 D to regenerate the plants with different hormonal combinations. Those of 0.1 mg/! of kinetine (Martinez, 1986) were prominent. The variability in the selection and evaluation processes is exposed in this paper.