Presenter Information

H Nakashima, Hokkaido University

Publication Date

1989

Description

Timothy (Phleum pratensis L.) is one of the most important grasses in Hokkaido, northern island of Japan, because of its winter hardiness and ability to give good quality hay. Recent advances in cellular and molecular biology of plant encourage to apply biotechnology not only to food crops but also to fod­der crops (Vasil, 1985). To create new breeding materials, somaclonal variation, embryo rescue from wide cross and pro­toplast fusion have been studied extensively. In vitro propa­gation is re-emphasized as a basic requirement for all aspects of plant biotechnology in relation to agriculture. This serial studies have been carried out to establish methods aiming to get regenerated plants from timothy seeds callus. At the moment, plantlets are only obtained from immature spikes cultures (Akashi and Ikeda, 1987). At first these experiments were car­ried out to understand the culture conditions of callus induc­tion, proliferation and regeneration on callus issued from tim­othy seeds.

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Tissue Culture of Timothy (Phleum pratensis L.)

Timothy (Phleum pratensis L.) is one of the most important grasses in Hokkaido, northern island of Japan, because of its winter hardiness and ability to give good quality hay. Recent advances in cellular and molecular biology of plant encourage to apply biotechnology not only to food crops but also to fod­der crops (Vasil, 1985). To create new breeding materials, somaclonal variation, embryo rescue from wide cross and pro­toplast fusion have been studied extensively. In vitro propa­gation is re-emphasized as a basic requirement for all aspects of plant biotechnology in relation to agriculture. This serial studies have been carried out to establish methods aiming to get regenerated plants from timothy seeds callus. At the moment, plantlets are only obtained from immature spikes cultures (Akashi and Ikeda, 1987). At first these experiments were car­ried out to understand the culture conditions of callus induc­tion, proliferation and regeneration on callus issued from tim­othy seeds.