Publication Date
1989
Description
The induction of non-physiological divisions in microspores through in vitro culture of anthers or pollen grains has led to the production of haploid plants in many species. However, there are still a large number of species in which haploid plants have not yet been obtained from generative cells. One of these species is Medicago sativa L., despite the great success attained in the regeneration of plants via tissue or protoplast culture. This work was conducted with the aim of studying the conditions to induce in vitro development in M. sativa microspores, as a first step in obtaining haploid plants through anther culture.
Citation
Piccirilli, M; Tanner, G J.; and Arcioni, S, "Polynucleate Pollen Formation in Cultured Medicago sativa L. Anthers" (2025). IGC Proceedings (1989-2023). 65.
https://uknowledge.uky.edu/igc/1989/session3b/65
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Polynucleate Pollen Formation in Cultured Medicago sativa L. Anthers
The induction of non-physiological divisions in microspores through in vitro culture of anthers or pollen grains has led to the production of haploid plants in many species. However, there are still a large number of species in which haploid plants have not yet been obtained from generative cells. One of these species is Medicago sativa L., despite the great success attained in the regeneration of plants via tissue or protoplast culture. This work was conducted with the aim of studying the conditions to induce in vitro development in M. sativa microspores, as a first step in obtaining haploid plants through anther culture.
