Publication Date

1989

Description

The induction of non-physiological divisions in microspores through in vitro culture of anthers or pollen grains has led to the production of haploid plants in many species. However, there are still a large number of species in which haploid plants have not yet been obtained from generative cells. One of these species is Medicago sativa L., despite the great success attained in the regeneration of plants via tissue or protoplast culture. This work was conducted with the aim of studying the cond­itions to induce in vitro development in M. sativa microspores, as a first step in obtaining haploid plants through anther culture.

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Polynucleate Pollen Formation in Cultured Medicago sativa L. Anthers

The induction of non-physiological divisions in microspores through in vitro culture of anthers or pollen grains has led to the production of haploid plants in many species. However, there are still a large number of species in which haploid plants have not yet been obtained from generative cells. One of these species is Medicago sativa L., despite the great success attained in the regeneration of plants via tissue or protoplast culture. This work was conducted with the aim of studying the cond­itions to induce in vitro development in M. sativa microspores, as a first step in obtaining haploid plants through anther culture.