Publication Date
1989
Description
Over 60 interspecific hybrids have been made in the genus Trifolium (Cleveland, 1985), some of them representing attempts to introduce persistance or pest resistance into cultivated clovers. Most of these hybrids, however, have been sterile. To attempt to restore fertility, colchicine methods have been applied to mature hybrid plants in vivo, but with limited success. A routine, simple technique for chromosome doubling in vegetative meristems is therefore still required, particularly for sterile hybrid materials which are available as mature vegetative clones rather than seed. We have adapted for use with clover hybrids the in vitro chromosome doubling technique used by Dore (1976) for Asparagus.
Citation
Moussett-Declas, Claire; Anderson, J A.; Williams, Elisabeth G.; and Taylor, N L., "An in Vitro Chromosome Doubling Method for Interspecific Hybrids of Trifolium" (2025). IGC Proceedings (1989-2023). 63.
https://uknowledge.uky.edu/igc/1989/session3b/63
Included in
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An in Vitro Chromosome Doubling Method for Interspecific Hybrids of Trifolium
Over 60 interspecific hybrids have been made in the genus Trifolium (Cleveland, 1985), some of them representing attempts to introduce persistance or pest resistance into cultivated clovers. Most of these hybrids, however, have been sterile. To attempt to restore fertility, colchicine methods have been applied to mature hybrid plants in vivo, but with limited success. A routine, simple technique for chromosome doubling in vegetative meristems is therefore still required, particularly for sterile hybrid materials which are available as mature vegetative clones rather than seed. We have adapted for use with clover hybrids the in vitro chromosome doubling technique used by Dore (1976) for Asparagus.
