Publication Date

1989

Description

The barriers to intergeneric crosses in the grass family were commonly known as inviability of the developing embryo, embryo abortion, or endosperm abnormalities, which might have occured after fertilization. To avoid incompatibility in wide cross, in vitro pollination or fertilization techniques have been attempted and made success in Nicotiana (Marubashi and Nakajima, 1985) and in Maize (Gengenbach, 1977). Our program has intended to improve forage quality of tall fescue through crossing to Italian ryegrass. The artificial cross between these genus had relatively low chance for obtaining hybrid offspring. The purpose of this research was to overcome the incompatibility barrier in intergeneric cross between Festuca genus and Lolium genus by in vitro pollination and culture and to develop hybrid offspring by ovule transfer.

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Intergeneric Hybridization Between Festuca and Lolium Genus by in vitro Pollination and by Ovule Transder

The barriers to intergeneric crosses in the grass family were commonly known as inviability of the developing embryo, embryo abortion, or endosperm abnormalities, which might have occured after fertilization. To avoid incompatibility in wide cross, in vitro pollination or fertilization techniques have been attempted and made success in Nicotiana (Marubashi and Nakajima, 1985) and in Maize (Gengenbach, 1977). Our program has intended to improve forage quality of tall fescue through crossing to Italian ryegrass. The artificial cross between these genus had relatively low chance for obtaining hybrid offspring. The purpose of this research was to overcome the incompatibility barrier in intergeneric cross between Festuca genus and Lolium genus by in vitro pollination and culture and to develop hybrid offspring by ovule transfer.