Lewis Honors College Capstone Collection

Year of Publication





Rehabilitation Sciences

Degree Name

Bachelor of Science in Education

First Capstone/Thesis Advisor

Dr. Charlotte Peterson


Muscle fiber type shifts in respect to satellite cells, muscle stem cells, are not well understood currently. The Peterson Lab has generated a mouse model (PAX7-DTA) that ablates satellite cells to determine if these muscle stem cells contribute to mouse muscle fiber type changes over an eight week period. In the study, control and satellite-cell-ablated mouse groups were split into control and overload groups (via synergist ablation surgery) and placed under similar environmental conditions. Eight weeks post-experiment, muscles were dissected to obtain the plantaris muscles of animals from all groups. Muscle cross-sections obtained were immunohistochemically stained, imaged, quantified by fiber type, and statistically analyzed. Results of the study found that in a two-way ANOVA analysis, there was no significant difference (P > 0.05) between groups. This result suggests that satellite cells play little apparent role in muscle fiber type shifts in mouse muscle in response to overload.