Date Available


Year of Publication


Degree Name

Master of Science (MS)

Document Type



Business and Economics


Pharmaceutical Sciences

First Advisor

Dr. Markos Leggas


Sterically stabilized liposomes with saturated lipid as the major lipid component (DSPC:m-PEGDSPE were applied in DB-67 delivery. The drug retention in vitro and pharmacokinetic properties in vivo were investigated. Liposomal DB-67 was cleared faster from the circulation in the larger liposomes (~180 nm) than in the smaller ones (~120 nm), even though DB-67 was retained longer in smaller size liposomes in vitro. Liposomal DB-67 clearance was increased when cholesterol was present in the liposomal composition (40 mole %). It can be attributable to the faster drug release from cholesterol containing liposomes as compared to liposomes without cholesterol. Cholesterol free liposomes with smaller particle size (~120 nm) were chosen as the optimal formulation. In addition, high lipid doses led to the lower clearance of liposomal DB-67 because the liposomal carriers were retained in the circulation longer. Liposomes of larger particle size were taken up by the liver and spleen to a greater extent than the smaller ones. But cholesterol content and lipid dose did not alter the tissue uptake of liposomes. The area under the DB-67 plasma concentration-time curve (AUC) for liposomal DB-67 was 40-fold higher that for non-liposomal DB-67.