We investigated alternatives to whole blood for blood feeding of mosquitoes with a focus on improved stability and compatibility with mass rearing programs. In contrast to whole blood, an artificial blood diet of ATP-supplemented plasma was effective in maintaining mosquito populations and was compatible with storage for extended periods refrigerated, frozen, and as a lyophilized powder. The plasma ATP diet supported rearing of both Anopheles and Aedes mosquitoes. It was also effective in rearing Wolbachia-infected Aedes mosquitoes, suggesting compatibility with vector control efforts.

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Published in PLOS Neglected Tropical Diseases, v. 11, 12, e0006142, p. 1-13.

© 2017 Baughman et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Funding was provided by The Global Good Fund I, LLC (www.globalgood.com) and the Liverpool Insect Testing Establishment via a grant from the Innovative Vector Control Consortium.

journal.pntd.0006142.s001.tif (121 kB)
S1 Fig. A primarily plasma-based diet supplemented with ATP supports egg development.

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