We investigated alternatives to whole blood for blood feeding of mosquitoes with a focus on improved stability and compatibility with mass rearing programs. In contrast to whole blood, an artificial blood diet of ATP-supplemented plasma was effective in maintaining mosquito populations and was compatible with storage for extended periods refrigerated, frozen, and as a lyophilized powder. The plasma ATP diet supported rearing of both Anopheles and Aedes mosquitoes. It was also effective in rearing Wolbachia-infected Aedes mosquitoes, suggesting compatibility with vector control efforts.
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Funding was provided by The Global Good Fund I, LLC (www.globalgood.com) and the Liverpool Insect Testing Establishment via a grant from the Innovative Vector Control Consortium.
Baughman, Ted; Peterson, Chelsea; Ortega, Corrie; Preston, Sarah R.; Paton, Christopher; Williams, Jessica; Guy, Amy; Omodei, Gavin; Johnson, Brian; Williams, Helen; O'Neill, Scott L.; Ritchie, Scott A.; Dobson, Stephen L.; and Madan, Damian, "A Highly Stable Blood Meal Alternative for Rearing Aedes and Anopheles Mosquitoes" (2017). Entomology Faculty Publications. 127.