US Patent Number
7345160
Publication Date
3-18-2008
Abstract
The invention relates to aequorin and obelin mutants whose emission is shifted with respect to wild type. The shift in emission is accomplished using a combination of mutations of amino acids within aequorin or obelin that affect bioluminescence; use of different types of chromophores, i.e., coelenterazines with variable emission characteristics; and modifications of the photoprotein with fluorophores that will allow for emission of light at longer wavelengths as a result of energy transfer. Additionally, an assay employing aequorin mutants to test for HIV-1 protease inhibitors is disclosed.
Assignees
University of Kentucky Research Foundation, Lexington, KY (US)
Application Number
10/811,138
Filing Date
03/29/2004
Recommended Citation
Daunert, Sylvia; Deo, Sapna K.; Dikici, Emre; and Rowe, Laura, "Aequorin and Obelin Mutants with Differing Wavelengths and Bioluminescence" (2008). Chemistry Faculty Patents. 13.
https://uknowledge.uky.edu/chemistry_patents/13
