The genome assembly of Anopheles darlingi consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among Anopheles species. Anopheles darlingi genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the An. darlingi scaffolds with homologous sequences of the Anopheles albimanus and Anopheles gambiae genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.
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This research was funded by Pronex/Rede Malaria from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico)/FAPEAM; CNPq/FAPEAM/INCT-ADAPTA II/INPA, Process number 465540/2014-7; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil, Financial Code 001; POSGRAD/FAPEAM 2017, Project # 002/2016 and PAPAC/FAPEAM 2019 (to J.S.B.); and the Russian State Budget Project #0324-2019-0042 (to M.V.S.).
The following are available online at https://www.mdpi.com/2075-4450/12/2/164/s1, Document S1: FASTA files of sequenced probes ends; Table S1: Blast results with size and length, and localization sites of scaffolds sequences of Anopheles darlingi, and synteny analysis with Anopheles albimanus and Anopheles gambiae; Table S2: List of genes located in the probes; Table S3: Genes IDs and functional annotation.
The above materials are also available for download as the additional file listed at the end of this record.
Rafael, Míriam Silva; Bridi, Leticia Cegatti; Sharakhov, Igor V.; Marinotti, Osvaldo; Sharakhova, Maria V.; Timoshevskiy, Vladimir A.; Guimarães-Marques, Giselle Moura; Santos, Valéria Silva; Nunes da Silva, Carlos Gustavo; Astolfi-Filho, Spartaco; and Tadei, Wanderli Pedro, "Physical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffolds" (2021). Biology Faculty Publications. 205.