Abstract
Metalloproteins bind and utilize metal ions for a variety of biological purposes. Due to the ubiquity of metalloprotein involvement throughout these processes across all domains of life, how proteins coordinate metal ions for different biochemical functions is of great relevance to understanding the implementation of these biological processes. Toward these ends, we have improved our methodology for structurally and functionally characterizing metal binding sites in metalloproteins. Our new ligand detection method is statistically much more robust, producing estimated false positive and false negative rates of ∼0.11% and ∼1.2%, respectively. Additional improvements expand both the range of metal ions and their coordination number that can be effectively analyzed. Also, the inclusion of additional quality control filters has significantly improved structure-function Spearman correlations as demonstrated by rho values greater than 0.90 for several metal coordination analyses and even one rho value above 0.95. Also, improvements in bond-length distributions have revealed bond-length modes specific to chemical functional groups involved in multidentation. Using these improved methods, we analyzed all single metal ion binding sites with Zn, Mg, Ca, Fe, and Na ions in the wwPDB, producing statistically rigorous results supporting the existence of both a significant number of unexpected compressed angles and subsequent aberrant metal ion coordination geometries (CGs) within structurally known metalloproteins. By recognizing these aberrant CGs in our clustering analyses, high correlations are achieved between structural and functional descriptions of metal ion coordination. Moreover, distinct biochemical functions are associated with aberrant CGs versus nonaberrant CGs.
Document Type
Article
Publication Date
3-7-2017
Digital Object Identifier (DOI)
https://doi.org/10.1002/prot.25257
Funding Information
Grant sponsor: National Science Foundation; Grant number: NSF 1252893 (Hunter N.B. Moseley, PI); Grant sponsor: National Institutes of Health; Grant number: NIH 1U24DK097215-01A1 (Richard M. Higashi, Teresa W.M. Fan, Andrew N. Lane, and Hunter N.B. Moseley, MPI); Grant number: P20GM103436 (Nigel Cooper, PI).
Related Content
Additional Supporting Information may be found in the online version of this article.
Software and full results available at: http://software.cesb.uky.edu and https://doi.org/10.6084/m9.figshare.4229297
Repository Citation
Yao, Sen; Flight, Robert M.; Rouchka, Eric C.; and Moseley, Hunter N. B., "Aberrant Coordination Geometries Discovered in the Most Abundant Metalloproteins" (2017). Molecular and Cellular Biochemistry Faculty Publications. 117.
https://uknowledge.uky.edu/biochem_facpub/117
Supporting Information
Included in
Amino Acids, Peptides, and Proteins Commons, Biochemistry, Biophysics, and Structural Biology Commons
Notes/Citation Information
Published in Proteins: Structure, Function and Bioinformatics, v. 85, issue 5, p. 885-907.
© 2017 The Authors Proteins: Structure, Function and Bioinformatics Published by Wiley Periodicals, Inc.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.