The increasing concern for food safety has created a need to evaluate novel techniques to eliminate or control pathogens, resulting in safe food. In this study, four bacteriophages of bovine origin, specific to E. coli O157:H7, were successfully isolated and characterized. A microplate reader assay demonstrated the efficacy of the bacteriophage (phage) cocktail against E. coli O157:H7 resulting in a significant reduction (p < 0.01) in the target pathogen population. The phage cocktail demonstrated significant efficacy (p < 0.05) against E. coli O157:H7 in the presence of the most utilized sanitizers in the United States, namely 100 parts per million (ppm) free chlorine and 100-ppm peroxyacetic acid. Survival in the sanitizer concentrations demonstrates the potential use of phage cocktail and sanitizer synergistically to enhance sanitation operations in the food industry.
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This work was supported by the Food Safety Outreach Competitive Grants Program, Grant No. 2017-70020-27246 from the USDA National Institute of Food and Agriculture.
Jagannathan, Badrinath Vengarai; Kitchens, Steven; Vijayakumar, Paul Priyesh; Price, Stuart; and Morgan, Melissa, "Potential for Bacteriophage Cocktail to Complement Commercial Sanitizer Use on Produce against Escherichia coli O157:H7" (2020). Animal and Food Sciences Faculty Publications. 28.