Abstract
Bacteriocin-producing (Bac+) lactic acid bacteria (LAB) comprising selected strains of Lactobacillus curvatus, Lactococcus lactis, Pediococcus acidilactici, and Enterococcus faecium and thailandicus were examined for inhibition of Listeria monocytogenes during hotdog challenge studies. The Bac+ strains, or their cell-free supernatants (CFS), were grouped according to mode-of-action (MOA) as determined from prior studies. Making a mixture of as many MOAs as possible is a practical way to obtain a potent natural antimicrobial mixture to address L. monocytogenes contamination of RTE meat products (i.e., hotdogs). The heat resistance of the bacteriocins allowed the use of pasteurization to eliminate residual producer cells for use as post-process surface application or their inclusion into hotdog meat emulsion during cooking. The use of Bac+ LAB comprising 3× MOAs directly as co-inoculants on hotdogs was not effective at inhibiting L. monocytogenes. However, the use of multiple MOA Bac+ CFS mixtures in a variety of trials demonstrated the effectiveness of this approach by showing a > 2-log decrease of L. monocytogenes in treatment samples and 6–7 log difference vs. controls. These data suggest that surface application of multiple mode-of-action bacteriocin mixtures can provide for an Alternative 2, and possibly Alternative 1, process category as specified by USDA-FSIS for control of L. monocytogenes on RTE meat products.
Document Type
Article
Publication Date
3-14-2017
Digital Object Identifier (DOI)
https://doi.org/10.3390/foods6030022
Funding Information
This paper was funded in part by an OCAST-OARS grant (#AR12-049), the Nutrition Physiology Co. (Guymon, OK, USA), the Dept. of Animal Science (Advance Foods-Gilliland Professorship), the R.M. Kerr Food & Ag Products Center, the USDA National Institute of Food and Agriculture, [Hatch Project #OKL02885], and the Division of Agricultural Sciences and Natural Resources at Oklahoma State University.
Repository Citation
Vijayakumar, Paul Priyesh and Muriana, Peter M., "Inhibition of Listeria monocytogenes on Ready-to-Eat Meats Using Bacteriocin Mixtures Based on Mode-of-Action" (2017). Animal and Food Sciences Faculty Publications. 11.
https://uknowledge.uky.edu/animalsci_facpub/11
Notes/Citation Information
Published in Foods, v. 6, issue 3, 22, p. 1-13.
© 2017 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).