Author ORCID Identifier

https://orcid.org/0000-0002-5617-8356

Year of Publication

2018

Degree Name

Master of Science (MS)

Document Type

Master's Thesis

College

Agriculture, Food and Environment

Department

Animal and Food Sciences

First Advisor

Dr. Surendranath P. Suman

Abstract

The effects of dietary ingredients on the proteome profile of postmortem beef longissimus lumborum (LL) muscle were evaluated. In the first experiment, the influence of dietary ractopamine on the whole-muscle proteome of beef LL was examined. Five proteins were differentially abundant between ractopamine-fed (RAC) and non-ractopamine fed (CON) groups. The differentially abundant proteins were over-abundant in RAC and were related to muscle structure development (F-actin-capping protein subunit beta-2 and PDZ and LIM domain protein-3), chaperone (heat shock protein beta-1), oxygen transportation (myoglobin), and glycolysis (L-lactate dehydrogenase A chain). These findings indicated that ractopamine influences the abundance of proteins associated with muscle structure and fiber type shift in beef LL.

In the second experiment, the effect of Vitamin E supplementation on the sarcoplasmic proteome of beef LL was characterized. Five differentially abundant proteins were observed between vitamin E-supplemented (VITE) and non-vitamin E-supplemented (CONT) groups. All the differentially proteins were over-abundant in CONT and were associated with antioxidant activity (thioredoxin-dependent peroxide reductase, peroxiredoxin-6, and serum albumin) and glycolysis (beta-enolase and triosephosphate isomerase). These results indicated that the strong antioxidant activity of vitamin E leads to low expression of antioxidant proteins and antioxidant-related glycolytic enzymes in beef LL muscle.

Digital Object Identifier (DOI)

https://doi.org/10.13023/ETD.2018.007

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