This study investigates a method using novel hybrid diffuse optical spectroscopies [near-infrared spectroscopy (NIRS) and diffuse correlation spectroscopy (DCS)] to obtain continuous, noninvasive measurement of absolute blood flow (BF), blood oxygenation, and oxygen consumption rate (V̇O2) in exercising skeletal muscle. Healthy subjects (n=9) performed a handgrip exercise to increase BF and V̇O2 in forearm flexor muscles, while a hybrid optical probe on the skin surface directly monitored oxy-, deoxy-, and total hemoglobin concentrations ([HbO2], [Hb], and THC), tissue oxygen saturation (StO2), relative BF (rBF), and relative oxygen consumption rate (rV̇O2). The rBF and rV̇O2 signals were calibrated with absolute baseline BF and V̇O2 obtained through venous and arterial occlusions, respectively. Known problems with muscle-fiber motion artifacts in optical measurements during exercise were mitigated using a novel gating algorithm that determined muscle contraction status based on control signals from a dynamometer. Results were consistent with previous findings in the literature. This study supports the application of NIRS/DCS technology to quantitatively evaluate hemodynamic and metabolic parameters in exercising skeletal muscle and holds promise for improving diagnosis and treatment evaluation for patients suffering from diseases affecting skeletal muscle and advancing fundamental understanding of muscle and exercise physiology.
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We thank the National Institutes of Health for support from NIH-R21AG034279.
This article was corrected in an Errata, and that Errata is attached to this record as Supplementary Content.
Katelyn Gurley, Yu Shang, and Guoqiang Yu, "Errata: Noninvasive optical quantification of absolute blood flow, blood oxygenation, and oxygen consumption rate in exercising skeletal muscle", Journal of Biomedical Optics 17(7), 079802 (2012).
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Gurley, Katelyn; Shang, Yu; and Yu, Guoqiang, "Noninvasive Optical Quantification of Absolute Blood Flow, Blood Oxygenation, and Oxygen Consumption Rate in Exercising Skeletal Muscle" (2012). Biomedical Engineering Faculty Publications. Paper 6.