US Patent Number
A hybrid vector carrying a first and second DNA segments operationally linked thereto, the first DNA segment encoding a protein capable of cross-linking to the cap structure of mRNA and mediating ribosome-binding, and the second DNA segment encoding a polypeptide or protein, the vector being capable of replication, transcription and translation to express the factor and the polypeptide or protein upon transformation of a eukaryotic host, and the polypeptide or protein being expressed at a level higher than the level of expression thereof in the absence of the first DNA segment. A eukaryotic host is transformed with this hybrid vector. Also disclosed is a method of increasing the synthesis of a polypeptide or protein in a eukaryotic host cell.
University of Kentucky Research Foundation, Lexington, Ky.
Rhoads, Robert E. and Benedetti, Arrico De, "Hybrid Vector and Method Resulting in Protein Overproduction by Eukaryotic Cells" (1997). Molecular and Cellular Biochemistry Faculty Patents. Paper 18.